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MMGG - Bulk Substrate Growing PDF Drucken E-Mail
Geschrieben von: MMGG   
Montag, 19. Januar 2009 um 23:06 Uhr
Beitragsseiten
MMGG
Overview of the Procedure
Preparation of the Substrate
Construction of the Terrarium
Poor Man's Terrarium
Basic Growing Chamber
Method 1: Hand Spraying
Method 2: Using a Fish Tank Bubblier
Method 3: Using an Ultra Sonic Humidifier
The Growing Cycle
Drying and Preservation of the Mushrooms
Dosage and Various. Ways to Ingest
Mushroom Juice
Mushroom Tea
Mushroom Soup
Mushroom Pizza
How to Get a Spore Syringe
Sources for a Spore Syringe
Sources for Spore Prints
How to Make a Spore Syringe
How to Make a Spore Print
Combination of Spore Print and Preparation of Inoculant
The Author's First Trip
Bulk Substrate Growing
Where to find Difficult Items
Brown Rice Flour
Ultra Sonic Humidifiers
Vermiculite
10 cc. Syringes
Providing Feed Back and Getting Questions Answered
Index of Adaptations
Adaptation-1: Use of Big Substrate Jars.
Adaptation-2: Use of a Pressure Cooker to Sterilize.
Adaptation-3: Tight Packing of Substrate to Slow Colonization
Adaptation-4: Injection at Only 1 Site
Adaptation-5: Large amounts of Inoculate
Adaptation-6: 80 Degree Colonization Temperature
Adaptation-7: More Oxygen for Colonization
Adaptation-8: Taking the Rice Cake Out of the Jar Before it is 100% Colonized
Adaptation-9: Waiting to Remove Rice Cake From the Jar
Adaptation-10: Checking Out Terrarium Prior to Removing Rice Cake
Adaptation-11: Use of a Coleman Cooler instead of a Styrofoam Cooler
Adaptation-12: Adding a Humidity Gauge to the Terrarium
Adaptation-13: Use of Fish Tank Heater to Raise Humidity
Adaptation-14: Use of Angel Hair to Raise Humidity
Adaptation-15: Supporting Extra Terrariums with the Ultra Sonic Humidifier
Adaptation-16: Use of an Ice-Pak to Initiate Fruiting
Adaptation-17: Adding Moisture to the Rice Cake
Adaptation-18: Continued Drying of Mushrooms During Storage
Adaptation-19: Reducing Output Of Ultra Sonic Humidifier
Adaptation-20: Providing a Moisture Reservoir in the Substrate
Adaptation-21: Use of a Timer to Control Suspended Water Droplets and Stimulate Fruiting
Adaptation-22: Use of a Home Made Brown Rice Flour
Adaptation-23: Lowering the Contamination Rate by Presterilization
Adaptation-24: Using a 2 1/2 gallon water jug for filtering
Adaptation-25: Insuring the Sterility of Caps Used for Spores
Adaptation-26: Producing a clean spore print from a questionable cap
Future Optimization
Alle Seiten

 

 



Bulk Substrate Growing


This section is for the mycrophile that has some experience and desires to cultivate a large crop of mushrooms. This section is intended for someone that has already grown several crops using the PF Tek procedure detailed earlier in this document. This section assumes a certain amount of experience and expertise. Please do not attempt this technique without first gaining the experience; you will most likely fail if you do so.

The procedure detailed earlier in this document is the best one known for making it possible for a first time cultivator to succeed. But it is very inefficient and the culture jars take a significant amount of time to manufacture and colonize. If you desire to grow a large quantity of mushrooms, you will find it is not practical for your needs.

The following procedure is an adaptation of the traditional mushroom cultivation techniques. It assumes you have some experience and don't need to be warned about this and that. The following description of the bulk growing procedure should be sufficient for an educated person to follow. There are many fine books on the subject and rather than compete with them, I suggest you get one if you start having difficulties The Mushroom Cultivator However, if you are careful, these directions should get you a truck load of shrooms.

There has been such a demand for instructions to accomplish growing on a bulk substrate, the author has decided to help. But the following process has not been 'idiot proofed' yet. You will need to use common sense and adapt as issues arise. Please note that this process depends on sterile procedures being in place. If you fail, it will most likely be because you introduced contaminates.


Materials needed:

  • 1 quart canning jars
  • Whole Grain Rye (Organically Grown)
  • Fresh Psilocybe Cubensis Mushrooom or 100% colonized culture jar
  • Pressure Cooker
  • Food Processor
  • 1 Quart canning jars
  • Coarse Ground Vermiculite
  • Large Cake or Roasting Pans
  • Mixing bowl

Step 1

The first step in the process is to generate several spawn jars. Place 2/3 cup of Rye in each canning jar. Add 3/4 cup of water to each jar. Loosely screw on the caps and sterilize the jars in the pressure cooker. 45 minutes at 15 PSI is usually good. Note that a pressure cooker is necessary for several reasons. First, bulk grain is more difficult to sterilize. Secondly, in order to get the grain to absorb as much water as possible, pressure is required.

As the Rye cooks, it will expand and all of the water should soak into the rye. Let the jars cool to room temperature. The rye should be loose and break apart if you rotate the jars. That is the main reason rye is used instead of some other grain. This will be important later.


Step 2

At this point you need mycelium to inoculate the grain. You will need a clean food processor and set of blades for it. If you can sterilize it in the pressure cooker, this is best. If not, throw it in the dish washer with lots of soap. Don't open the pressure cooker or dish washer until you are ready to use it.

If you are using a mushroom, perform the following steps. Sterilize 1/4 cup of water. Let it cool. Put the water and the mushroom into the food processor and turn the mixture into a slurry. Don't mix the slurry more than you need to. You are creating small fragments of mycelium by chopping up the mushroom. The more you chop it up, the more damage you do to the small pieces of mycelium.

Open each spawn jar and place 4 or 5 cc's of the prepared slurry in the jar. Close the lid and rotate the jar so the Rye kernels tumble and mix inside the jar. The idea is to get as many kernels as possible to have mycelium fragments on them. Loosen the lid and place the jars in a warm, dark location.

There are several reasons why tissue from a mushroom is preferred to spores for the inoculation of the spawn jars.

  • Mycelium does not need to germinate. It starts growing immediately.
  • Normally only 1/3 of the diakratic tissue in a multi-spore inoculation is capable of fruiting. By defination, this tissue came from a fruit body and is capable of fruiting.
  • Since every mushroom grown on the bulk substrate is of identical genetic origin, they will be very close in potency.

If you are using a 100% colonized culture jar, perform the following steps. First, the culture jar should have been 100% colonized for at least a week. This lets the mycelium grow into the cake and results in more of it being available for the inoculation. Birth the cake, and place it in the food processor. Turn it into little pieces smaller than a pea. Dump the sterilized rye into the food processor and turn it on just long enough to mix things up well. Place the contents of the food processor back into the jar and cover it loosely.

The cleaner your environment the more likely you will get through this stage without introducing contaminates. This is the most likely place to induce failure.


Step 3

Loosen the lid and place the jars in a warm, dark location. In 3 or 4 days you should see isolated spots with white mycelium growing. Check the spawn jars periodically. When you see large areas of aggressive growth, tumble the Rye to mix things up. Rotate the jar enough to thoroughly mix the kernels. The idea of mixing the Rye is to get kernels with mycelium growing on them scattered throughout the jar. Ideally, no kernel should be too far from a kernel with mycelium growing on it. Repeat this process every 3 or 4 days when the mycelium growth has been aggressive for a while. Once there is mycelium growing within 1/2 inch of every other location no further mixing is needed. At this point just let the mycelium expand outwards until it is every where.


Step 4

Once every kernel has mycelium growing on it, leave the jar sit undisturbed for a week. The idea is to let the mycelium grow as fast as possible.


Step 5

Sterilize more jars filled with rye and water. Let the jars cool.


Step 6

Most food processors have a plastic blade that comes with them for the purpose of mixing things instead of cutting things. Use this if possible. Make sure your food processor and blade are clean. Empty the contents of a colonized spawn jar into the food processor. Turn it on until the Rye kernels are all broken apart. Add some of the freshly cooked Rye to the food processor. Depending upon your confidence and the sterility of your environment you can add anywhere from 4 to 20 times the amount of grain in your spawn jar. The less you add, the less likely you will have problems with contamination. The more you add, the faster you can create substrate. Initially, you should stay on the low side. Turn on the food processor and mix the freshly cooked Rye with the colonized Rye from the spawn jar.

Place this material in a container that can be loosely covered. This material should be treated exactly as the earlier spawn jars were treated except you should see quicker growth of the mycelium patches. The amount of spawn you have can be compounded again and again until you have enough to inoculate massive substrates.


Step 7

Once you have sufficient spawn colonized and available for your substrate, lay it out in a deep cake pan to a depth of 1.5 inches. Attempt to keep the surface even. If you have a very clean environment, you can perform a mass expansion and lay out the uncolonized grain instead of waiting for it to colonize in the jar. This will save several days and a little effort, but in general it is not worth the risk.

Cover the pan with a sheet of plastic wrap. Do not seal it absolutely tight, but make sure that very little air is exchanged. Note that some air does need to be exchanged to keep the CO2 level from getting too high. But air exchange increases the likelihood of contamination. Keep it to a minimum.

This is one of the big benefits to bulk substrate growing. Whatever grain is not used to prepare a substrate can be used as spawn for new jars. When you get to this point in the process, you can easily be preparing a new bulk substrate every couple of days. You just make sure you never use all of the colonized grain as substrate. You hold back some to use as spawn for the next set of jars.


Step 8

Wait a few days until the freshly laid out substrate is showing aggressive growth. Laying out the grain breaks up the mycelium networks, and it takes several days for the mycelium to recover.


Step 9

Casing the substrate is the next step. Various recipes are available but the simplest one consists of course ground vermiculite and water. Course ground vermiculite is a requirement because the fine ground vermiculite packs too tightly and seals the substrate.

Soak the vermiculite in water. Wring it out, but leave it fairly damp. Later versions of this guide will have exact measurements but for the moment, you will need to adapt. If any of you think you have an optimum mixture, please post it in the alt.drugs.psychedlics and rec.drugs.psychedlic newsgroups.

Lay out the casing material to a depth of 3/4 of an inch. Try to keep it smooth because this will result in the mycelium poking through everywhere at the same time. Cover with plastic wrap and wait. Typically, it will be about a week for the mycelium to break through the surface of the casing.


Step 10

Initiate fruiting. Take the plastic off of the pan and place it in your terrarium. Make sure you have the temperature at about 75 F. Have some indirect light available. As pins start to develop, use a hand sprayer to mist the casing and keep it moist. But be careful. Do not saturate it to the point of being wet and having water drops that will not soak into the vermiculite.


Step 11

After the first flush, you can get a smaller second and third flush if you let the substrate rest for a while. There are a lot of factors affecting this. One particularly important factor is home much of the first flush's moisture came from the substrate and how much came from the casing. If you can mist your casing several times a day, that will help the longevity of your substrate.


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